Difference between revisions of "Part:BBa K2548007"
Joycelalala (Talk | contribs) |
Joycelalala (Talk | contribs) |
||
| Line 5: | Line 5: | ||
aiiA coding device regulated by C4-HSL. | aiiA coding device regulated by C4-HSL. | ||
| − | <h2>Construction of Quorum Quenching Device II</h2> | + | <h2>Construction of Quorum Quenching Device II (BBa_K2548000 + BBa_K2548007)</h2> |
https://static.igem.org/mediawiki/parts/thumb/e/e6/Quorumquenchingdevice2.png/799px-Quorumquenchingdevice2.png | https://static.igem.org/mediawiki/parts/thumb/e/e6/Quorumquenchingdevice2.png/799px-Quorumquenchingdevice2.png | ||
<h2>Characterization of Quorum Quenching Device II</h2> | <h2>Characterization of Quorum Quenching Device II</h2> | ||
| − | [[Image:QQresults.png|thumb|center|400px|Fig. | + | [[Image:QQresults.png|thumb|center|400px|Fig.1 Quorum quenching results]]<br> |
<p>We did the experiment XIX to test the quorum quenching ability of QQ Device I (BBa_K2548000 + BBa_K2548006) and QQ Device II (BBa_K2548000 + BBa_K2548007), and the result is shown below. E. coli DH5a transformed with sensor device I was induced for 3 hours in the supernatant of cells containing empty RPG plasmid, QQ device I, and QQ device II after they were induced by C4-HSL for 4 hours. In Fig. 1, the control group with empty RPG plasmid shows higher GFP concentration than the culture of QQ device I and QQ device II, indicating that C4-HSL was consumed by the two devices. The GFP concentrations reduced 18.5% and 20.9% for device I and device II compared with control group respectively, implying that QQ device I had better C4-HSL degradation ability.</p> | <p>We did the experiment XIX to test the quorum quenching ability of QQ Device I (BBa_K2548000 + BBa_K2548006) and QQ Device II (BBa_K2548000 + BBa_K2548007), and the result is shown below. E. coli DH5a transformed with sensor device I was induced for 3 hours in the supernatant of cells containing empty RPG plasmid, QQ device I, and QQ device II after they were induced by C4-HSL for 4 hours. In Fig. 1, the control group with empty RPG plasmid shows higher GFP concentration than the culture of QQ device I and QQ device II, indicating that C4-HSL was consumed by the two devices. The GFP concentrations reduced 18.5% and 20.9% for device I and device II compared with control group respectively, implying that QQ device I had better C4-HSL degradation ability.</p> | ||
Latest revision as of 21:21, 17 October 2018
Prhl(LR)-RBS-aiiA-Terminator
aiiA coding device regulated by C4-HSL.
Construction of Quorum Quenching Device II (BBa_K2548000 + BBa_K2548007)
Characterization of Quorum Quenching Device II
We did the experiment XIX to test the quorum quenching ability of QQ Device I (BBa_K2548000 + BBa_K2548006) and QQ Device II (BBa_K2548000 + BBa_K2548007), and the result is shown below. E. coli DH5a transformed with sensor device I was induced for 3 hours in the supernatant of cells containing empty RPG plasmid, QQ device I, and QQ device II after they were induced by C4-HSL for 4 hours. In Fig. 1, the control group with empty RPG plasmid shows higher GFP concentration than the culture of QQ device I and QQ device II, indicating that C4-HSL was consumed by the two devices. The GFP concentrations reduced 18.5% and 20.9% for device I and device II compared with control group respectively, implying that QQ device I had better C4-HSL degradation ability.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 172
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 106
Illegal NgoMIV site found at 695 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 614