Difference between revisions of "Part:BBa K2550001:Design"
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===Source===
 
===Source===
  
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Collins Paper
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Alexander A. Green, Pamela A. Silver, James J. Collins, Peng Yin, Toehold Switches: De-Novo-Designed Regulators of Gene Expression, Cell, Volume 159, Issue 4,
 +
2014, Pages 925-939, ISSN 0092-8674, https://doi.org/10.1016/j.cell.2014.10.002
  
 
===References===
 
===References===

Revision as of 21:21, 17 October 2018


T7 promoter with a trigger sequence to match the toehold sequence of part BBa_K2550000


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 104
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

The RNA sequence was obtained from the Georgia Institue of Technology that was number 2 of the 144 generation orthogonal toehold switch collection from the Collins Paper titled, Toehold Switches: De-Novo-Designed Regulators of Gene Expression.

Design Notes

This is meant to be used with toehold part. Needs to be used in BL21 cells that have a T7 polymerase.


Source

Alexander A. Green, Pamela A. Silver, James J. Collins, Peng Yin, Toehold Switches: De-Novo-Designed Regulators of Gene Expression, Cell, Volume 159, Issue 4, 2014, Pages 925-939, ISSN 0092-8674, https://doi.org/10.1016/j.cell.2014.10.002

References