Difference between revisions of "Part:BBa K2624007:Experience"

Revision as of 21:08, 17 October 2018

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2624007

We used this part to construct the "p-H*" plasmid and "p-H*-U6" plasmid, in order to verify the role of the RNA interference caused by pri-miRNA. Through qPCR experiment, it can be seen that the miRNA expression level of MAP4K4 is increased.

20181017210154%21T--CPU_CHINA--hp-qidongzi.png

Figure. The expression of miRNA(MAP4K4)

"p-H*" is "pcDNA-HULC--primiRNA" plasmid; "p-H*-U6" is "pcDNA-HULC-primiRNA-U6-pSil" plasmid;

User Reviews

UNIQ476a85394fd1dca6-partinfo-00000000-QINU UNIQ476a85394fd1dca6-partinfo-00000001-QINU

@@ Line 9: / Line 9: @@
 https://static.igem.org/mediawiki/2018/archive/5/56/20181017210154%21T--CPU_CHINA--hp-qidongzi.png
-Figure. The expression of miRNA(MAP4K4) (A) and MAP4K4 mRNA (B)
+Figure. The expression of miRNA(MAP4K4)
 "p-H*" is "pcDNA-HULC--primiRNA" plasmid; "p-H*-U6" is "pcDNA-HULC-primiRNA-U6-pSil" plasmid;