Difference between revisions of "Part:BBa K2624007:Experience"
(→‎Applications of BBa_K2624007)
Line 5: Line 5:
  
 
===Applications of BBa_K2624007===
 
===Applications of BBa_K2624007===
−
We used this part to construct the "p-H" plasmid in order to verify the role of RNA interference caused by pri-miRNA. According to the results of qPCR, the decreased MAP4K4 mRNA expression was observed.
+
We used this part to construct the "p-H*" plasmid and "p-H*-U6" plasmid, in order to verify the role of the RNA interference caused by pri-miRNA. Through qPCR experiment, it can be seen that the miRNA expression level of MAP4K4 is increased.
  
−
https://static.igem.org/mediawiki/2018/5/56/T--CPU_CHINA--hp-qidongzi.png
+
https://static.igem.org/mediawiki/2018/archive/5/56/20181017210154%21T--CPU_CHINA--hp-qidongzi.png
  
−
Figure. The expression of mRNA(MAP4K4)
+
Figure. The expression of miRNA(MAP4K4) (A) and MAP4K4 mRNA (B)
  
−
"p-H" is "pcDNA-HULC--primiRNA(MAP4K4)" plasmid;"Plasmid1" is"pIRES-TRE--NS5B-NLS";"Plasmid2" is"pcDNA-HULC--primiRNA(MAP4K4)-U6-pSil-TRE-sponge";
+
"p-H*" is "pcDNA-HULC--primiRNA" plasmid; "p-H*-U6" is "pcDNA-HULC-primiRNA-U6-pSil" plasmid;
  
 
===User Reviews===
 
===User Reviews===

Revision as of 21:08, 17 October 2018


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2624007

We used this part to construct the "p-H*" plasmid and "p-H*-U6" plasmid, in order to verify the role of the RNA interference caused by pri-miRNA. Through qPCR experiment, it can be seen that the miRNA expression level of MAP4K4 is increased.

20181017210154%21T--CPU_CHINA--hp-qidongzi.png

Figure. The expression of miRNA(MAP4K4) (A) and MAP4K4 mRNA (B)

"p-H*" is "pcDNA-HULC--primiRNA" plasmid; "p-H*-U6" is "pcDNA-HULC-primiRNA-U6-pSil" plasmid;

User Reviews

UNIQe9a46927a8005c3b-partinfo-00000000-QINU UNIQe9a46927a8005c3b-partinfo-00000001-QINU