Difference between revisions of "Part:BBa K2717013:Design"

(→‎Design Notes)
(→‎Source)
 
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===Source===
 
===Source===
  
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We obtained the sequence expressing the VHb protein from part BBa_K1321200 by PCR, and obtained the TorA signal sequence by gene synthesis. We linked these two sequences to the linearized vector by infusion. We placed the TorA sequence upstream of the VHb sequence and initiated expression by a promoter.
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We obtained the sequence expressing the VHb protein from <partinfo>BBa_K1321200</partinfo>(2014 Imperial), and obtained the TorA signal sequence by gene synthesis. We linked these two sequences to the linearized vector by infusion. We placed the TorA sequence upstream of the VHb sequence and initiated expression by a promoter.
  
 
===References===
 
===References===

Latest revision as of 20:40, 17 October 2018


TorA signal-VHb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 540
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added TorA sequence to the origin part.

Source

We obtained the sequence expressing the VHb protein from BBa_K1321200(2014 Imperial), and obtained the TorA signal sequence by gene synthesis. We linked these two sequences to the linearized vector by infusion. We placed the TorA sequence upstream of the VHb sequence and initiated expression by a promoter.

References