Difference between revisions of "Part:BBa K2596015:Design"

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===Source===
 
===Source===
  
−
BBa_K2596010 (RBS) from <i>Synechocystis sp.</i> 6803
+
BBa_K2596010 (RBS) from <i>Synechocystis sp.</i> PCC 6803
  
 
BBa_K2596005 (<i>cscB</i>) from <i>E. coli</i>
 
BBa_K2596005 (<i>cscB</i>) from <i>E. coli</i>

Latest revision as of 20:36, 17 October 2018


RBS + cscB (codon optimized for cyanobacteria)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 498
    Illegal NgoMIV site found at 621
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We made sure that the system would be efficient for cyanobacteria, so we used codon optimization and RBS native to cyanobacteria.


Source

BBa_K2596010 (RBS) from Synechocystis sp. PCC 6803

BBa_K2596005 (cscB) from E. coli

References

LdpA: a component of the circadian clock senses redox state of the cell. Ivleva NB, Bramlett MR, Lindahl PA, Golden SS. EMBO J. 2005 Mar 23;24(6):1202-10. Epub 2005 Mar 10. 10.1038/sj.emboj.7600606 PubMed 15775978