Difference between revisions of "Part:BBa K2839007"
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:This composite part was submitted by iGEM Thessaloniki and consists of Psp1w1 promoter driving the expression of sfGFP fluorescent marker. Psp1w1 promoter has a single operator located between the -35 and -10 elements, where TAL Effector 1 can tightly bind, decoupling its expression from plasmid/gene copy number. Copy number positively affects GOI expression, while its repressor, being under the same positive influence, compensates for this change resulting in the disassociation of the GOI’s expression from the plasmid’s copy number | :This composite part was submitted by iGEM Thessaloniki and consists of Psp1w1 promoter driving the expression of sfGFP fluorescent marker. Psp1w1 promoter has a single operator located between the -35 and -10 elements, where TAL Effector 1 can tightly bind, decoupling its expression from plasmid/gene copy number. Copy number positively affects GOI expression, while its repressor, being under the same positive influence, compensates for this change resulting in the disassociation of the GOI’s expression from the plasmid’s copy number | ||
| − | :Psp1w1 (B0032) device leads to a stronger sfGFP expression when compared to Psp1w1 B0030 device [[Part: | + | :Psp1w1 (B0032) device leads to a stronger sfGFP expression when compared to Psp1w1 B0030 device [[Part:BBa_K2839008]] due to the different RBS. RiboJ insulates the translational unit from surrounding sequences. |
This part is included in our Part Collection, a genetic toolbox for controlled gene expression. | This part is included in our Part Collection, a genetic toolbox for controlled gene expression. | ||
Latest revision as of 20:31, 17 October 2018
Insulated Psp1w1 promoter (B0032 rbs)
Short Description
- This composite part was submitted by iGEM Thessaloniki and consists of Psp1w1 promoter driving the expression of sfGFP fluorescent marker. Psp1w1 promoter has a single operator located between the -35 and -10 elements, where TAL Effector 1 can tightly bind, decoupling its expression from plasmid/gene copy number. Copy number positively affects GOI expression, while its repressor, being under the same positive influence, compensates for this change resulting in the disassociation of the GOI’s expression from the plasmid’s copy number
- Psp1w1 (B0032) device leads to a stronger sfGFP expression when compared to Psp1w1 B0030 device Part:BBa_K2839008 due to the different RBS. RiboJ insulates the translational unit from surrounding sequences.
This part is included in our Part Collection, a genetic toolbox for controlled gene expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 710
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]