Difference between revisions of "Part:BBa K2577006"

 
 
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antifreeze and antimicrobial protein IAFGP regulated by AraC.
 
antifreeze and antimicrobial protein IAFGP regulated by AraC.
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 +
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<center>https://static.igem.org/mediawiki/2018/6/68/T--WPI_Worcester--COncentrationofProtein.png</center>
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This graph shows the concentrations of purified proteins, including IAFGP, labeled as "IA" on the graph.
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<center>https://static.igem.org/mediawiki/2018/d/da/T--WPI_Worcester--CoatedPlates.png</center>
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<center>https://static.igem.org/mediawiki/2018/0/0e/T--WPI_Worcester--MixedPlate.png</center>
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These are graphs of biofilm assays that included the protein IAFGP. The proteins were either mixed into the bacteria or it was used to coat the plate that the biofilm was going to be grown on.
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<center>https://static.igem.org/mediawiki/2018/b/bf/T--WPI_Worcester--Bioflim_Assay_Plate.png</center>
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This is a graph of the biofilm production with and without the expression of different antifreeze proteins. For this biofilm assay, an AraC promoter was used to regulate the protein expression.
 +
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The expression of these proteins was proven on the following gel:
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<center>https://static.igem.org/mediawiki/2018/7/70/T--WPI_Worcester--ProteinGel1.png</center>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 20:24, 17 October 2018


AraC regulated IAFGP

antifreeze and antimicrobial protein IAFGP regulated by AraC.


T--WPI_Worcester--COncentrationofProtein.png

This graph shows the concentrations of purified proteins, including IAFGP, labeled as "IA" on the graph.

T--WPI_Worcester--CoatedPlates.png
T--WPI_Worcester--MixedPlate.png

These are graphs of biofilm assays that included the protein IAFGP. The proteins were either mixed into the bacteria or it was used to coat the plate that the biofilm was going to be grown on.

T--WPI_Worcester--Bioflim_Assay_Plate.png

This is a graph of the biofilm production with and without the expression of different antifreeze proteins. For this biofilm assay, an AraC promoter was used to regulate the protein expression.

The expression of these proteins was proven on the following gel:

T--WPI_Worcester--ProteinGel1.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1367
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1307
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]