Difference between revisions of "Part:BBa K2719007:Experience"

Revision as of 20:23, 17 October 2018

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Please enter how you used this part and how it worked out.

Applications of BBa_K2719007

This part was cloned on pSB1C3 using EcoRI and PstI, later it was transformed on E.coli DH5a (Figure 1).

Figure 1. Colonies transformed with Collagen V-like expression device, the red strain indicates a successful clonation and transformation of the plasmid

Then the plasmid was extracted and run on an agarose gel electrophoresis (Figure 2). Later an restriction was performed using NotI and was run on an agarose gel (Figure 3) to probe the presence of the insert. Later the extracted plasmid was transformed on E.coli Rossetta (DE3).

Figure 2. 0.85% agarose gel with GelRed, MW)NEB 1Kb Plus DNA Ladder, 9)Extraction Collagen V-like (BBa_K2719007), 10)Extraction Collagen V-like (BBa_K2719007) duplicate.

User Reviews

UNIQ0bcb080a6ab21dfc-partinfo-00000000-QINU UNIQ0bcb080a6ab21dfc-partinfo-00000001-QINU

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 <p>Then the plasmid was extracted and run on an agarose gel electrophoresis (Figure 2).  Later an restriction was performed using NotI and was run on an agarose gel (Figure 3) to probe the presence of the insert. Later the extracted plasmid was transformed on <i>E.coli</i> Rossetta (DE3).</p>
+[[file:T--TecCEM--GelK2719007.png|500px]]
+<p><i>Figure 2.</i> 0.85% agarose gel with GelRed, MW)NEB 1Kb Plus DNA Ladder, 9)Extraction Collagen V-like (BBa_K2719007), 10)Extraction Collagen V-like (BBa_K2719007) duplicate.</p>
 ===User Reviews===
 <!-- DON'T DELETE --><partinfo>BBa_K2719007 StartReviews</partinfo>