Difference between revisions of "Part:BBa K2656123"

Revision as of 20:22, 17 October 2018

pT7-GFPmut3b transcriptional unit

Transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease (Figure 1).

Figure 1. Transcriptional unit assembled. Golden Gate scars: standardized overhangs for promoter, RBS, CDS and terminator

This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

BBa_K2656000: the BBa_I719005 constitutive strong promoter in its Golden Braid compatible version. Promoter T7 leads into a high protein expression.
BBa_K2656010: the B0032 low ribosome biding site in its Golden Braid compatible version
BBa_K2656022: the BBa_K2656022 GFPmut3b sequence in its Golden Braid standardized version
Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 Transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease (Figure 1).
-[[File:T--Valencia_UPV_TU_UPV2018.png|500px|thumb|none|alt=TU.|Figure 1. Transcriptional unit assembled. Golden Gote scars: standardized overhangs for promoter, RBS, CDS and terminator]]
+[[File:T--Valencia_UPV_TU_UPV2018.png|500px|thumb|none|alt=TU.|Figure 1. Transcriptional unit assembled. Golden Gate scars: standardized overhangs for promoter, RBS, CDS and terminator]]
 This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]: