Difference between revisions of "Part:BBa K2717012:Design"

 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
rbs2000-GFP-hairpin-gdh-tag
+
The glucose dehydrogenase is isolated from the genome of the laboratory strain. V5 tag and His tag are obtained from our own lab. The sequence of rbs2000 is obtained from the Kit and we synthesize by ourselves. The hairpin structure is synthesize by ourselves.
 +
 
  
 
===References===
 
===References===

Revision as of 20:15, 17 October 2018


RBS2000-GFP-hairpin-GDH-v5 tag-his tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2336
    Illegal AgeI site found at 3228
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3210
    Illegal BsaI.rc site found at 678


Design Notes

rbs2000-GFP-hairpin-gdh-tag


Source

The glucose dehydrogenase is isolated from the genome of the laboratory strain. V5 tag and His tag are obtained from our own lab. The sequence of rbs2000 is obtained from the Kit and we synthesize by ourselves. The hairpin structure is synthesize by ourselves.


References