Difference between revisions of "Part:BBa K2624000:Experience"

Line 7: Line 7:
 
To improve the selectivity of our therapy system, we utilize hTERT, the cancer-specific promoter, and HULC, the HCC-specific promoter, to open an AND-gated system to target HCC (Hepatocellular carcinoma). pGL3-Basic vector, a promoterless vector is used for measuring the activity of promoter with a luciferase assay. We determined the activity of the two promoters by it. Experimental results show that the promoter has a good startup efficiency.
 
To improve the selectivity of our therapy system, we utilize hTERT, the cancer-specific promoter, and HULC, the HCC-specific promoter, to open an AND-gated system to target HCC (Hepatocellular carcinoma). pGL3-Basic vector, a promoterless vector is used for measuring the activity of promoter with a luciferase assay. We determined the activity of the two promoters by it. Experimental results show that the promoter has a good startup efficiency.
 
|width="40%"|
 
|width="40%"|
"https://static.igem.org/mediawiki/2018/5/56/T--CPU_CHINA--hp-qidongzi.png"</center>
+
"https://static.igem.org/mediawiki/2018/5/56/T--CPU_CHINA--hp-qidongzi.png"
  
 
===User Reviews===
 
===User Reviews===

Revision as of 20:15, 17 October 2018


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2624000

To improve the selectivity of our therapy system, we utilize hTERT, the cancer-specific promoter, and HULC, the HCC-specific promoter, to open an AND-gated system to target HCC (Hepatocellular carcinoma). pGL3-Basic vector, a promoterless vector is used for measuring the activity of promoter with a luciferase assay. We determined the activity of the two promoters by it. Experimental results show that the promoter has a good startup efficiency. |width="40%"| "T--CPU_CHINA--hp-qidongzi.png"

User Reviews

UNIQ7bcdc31bc4464d11-partinfo-00000000-QINU UNIQ7bcdc31bc4464d11-partinfo-00000001-QINU