Difference between revisions of "Part:BBa K2765052:Experience"

Latest revision as of 20:07, 17 October 2018

We ligated the promoter to roGFP2-Orp1 by Overlap Extension PCR and ligated it to the plasmid pESC-Trp containing the terminator cyc1 by enzyme digestion.

T--BIT-China--iGEM2018-PartsOutput-T--BIT-China--iGEM2018-PartsOutput-ENO2ligated.png

After sequencing verification, we transferred the correct plasmid into yeast for expression. Then we measured the fluorescence intensity of roGFP2-Orp1(Excitation wavelength is 488nm and the emission is followed at 515nm), which improved that the part can work properly.

T--BIT-China--iGEM2018-PartsOutput-T--BIT-China--iGEM2018-PartsOutput-ENO2experiment.png

Applications of BBa_K2765052

User Reviews

UNIQ4e3dbb40a9d38f7c-partinfo-00000000-QINU UNIQ4e3dbb40a9d38f7c-partinfo-00000001-QINU

@@ Line 1: / Line 1: @@
 __NOTOC__
-This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
+We ligated the promoter to roGFP2-Orp1 by Overlap Extension PCR and ligated it to the plasmid pESC-Trp containing the terminator cyc1 by enzyme digestion.
-how you used this part and how it worked out.
+[[Image: T--BIT-China--iGEM2018-PartsOutput-T--BIT-China--iGEM2018-PartsOutput-ENO2ligated.png |center|300px|]]
+After sequencing verification, we transferred the correct plasmid into yeast for expression. Then we measured the fluorescence intensity of roGFP2-Orp1(Excitation wavelength is 488nm and the emission is followed at 515nm), which improved that the part can work properly.
+[[Image: T--BIT-China--iGEM2018-PartsOutput-T--BIT-China--iGEM2018-PartsOutput-ENO2experiment.png |center|400px|]]
 ===Applications of BBa_K2765052===