Difference between revisions of "Part:BBa K2717010"

Latest revision as of 20:05, 17 October 2018

gdh-gfp-reverse

In this composite part, we link GFP and glucose dehydrogenase in reverse and use Promoter J23100 and RBS 2000 control the GFP and Promoter lacI control the glucose dehydrogenase and tag. Since GFP and gdh are arranged in reverse, the two genes express independently and don’t affect each other.

Since GFP and gdh are arranged in reverse and there are two terminators between them, the two genes express independently and don’t affect each other. We designed this part as a comparison to confirm that the introduction of TGATG allows the plasmid to highly express the gene of interest (the upstream gene of TGATG).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1157
Illegal AgeI site found at 2049
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 644
Illegal BsaI.rc site found at 1175

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 <partinfo>BBa_K2717010 short</partinfo>
-gdh-gfp-&#21453;&#21521;
+In this composite part, we link GFP and glucose dehydrogenase in reverse and use Promoter J23100 and RBS 2000 control the GFP and Promoter lacI control the glucose dehydrogenase and tag. Since GFP and gdh are arranged in reverse, the two genes express independently and don’t affect each other.
+Since GFP and gdh are arranged in reverse and there are two terminators between them, the two genes express independently and don’t affect each other. We designed this part as a comparison to confirm that the introduction of TGATG allows the plasmid to highly express the gene of interest (the upstream gene of TGATG).
 <!-- Add more about the biology of this part here