Difference between revisions of "Part:BBa K2656123"
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<partinfo>BBa_K2656123 short</partinfo> | <partinfo>BBa_K2656123 short</partinfo> | ||
| − | Transcriptional unit | + | Transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease (Figure 1). |
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| + | [[File:T--Valencia_UPV_TU_UPV2018.png|500px|thumb|none|alt=TU.|Figure 1. Transcriptional unit assembled. Golden Gote scars: standardized overhangs for promoter, RBS, CDS and terminator]] | ||
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| + | This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]: | ||
| + | <html> | ||
| + | <ul> | ||
| + | <li></html>[https://parts.igem.org/Part:BBa_BBa_K2656000 BBa_K2656000]: the [https://parts.igem.org/Part:BBa_BBa_I719005 BBa_I719005] constitutive strong promoter in its Golden Braid compatible version <html></li> | ||
| + | <li></html>[https://parts.igem.org/Part:BBa_BBa_K2656010 BBa_K2656010]: the [https://parts.igem.org/Part:BBa_B0032 B0032] low ribosome biding site in its Golden Braid compatible version <html></li> | ||
| + | <li></html> [https://parts.igem.org/Part:BBa_K2656022 BBa_K2656022]: the [https://parts.igem.org/Part:BBa_K2656022 BBa_K2656022] GFPmut3b sequence in its Golden Braid standardized version <html></li> | ||
| + | <li></html> Terminator [https://parts.igem.org/Part:BBa_K2656026 BBa_K2656026]: the [https://parts.igem.org/Part:BBa_B0015 B0015] transcriptional terminator in its Golden Braid compatible version<html></li> | ||
| + | </ul> </html> | ||
Revision as of 20:03, 17 October 2018
pT7-GFPmut3b transcriptional unit
Transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease (Figure 1).
This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:
- BBa_K2656000: the BBa_I719005 constitutive strong promoter in its Golden Braid compatible version
- BBa_K2656010: the B0032 low ribosome biding site in its Golden Braid compatible version
- BBa_K2656022: the BBa_K2656022 GFPmut3b sequence in its Golden Braid standardized version
- Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]