Difference between revisions of "Part:BBa K2765051:Experience"

 
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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We ligated the promoter to roGFP2-Orp1 by Overlap Extension PCR and ligated it to the plasmid pESC-Trp containing the terminator cyc1 by enzyme digestion.
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how you used this part and how it worked out.
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[[Image: T--BIT-China--iGEM2018-PartsOutput-TEF1ligated.png |center|300px|]]
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After sequencing verification, we transferred the correct plasmid into yeast for expression. Then we measured the fluorescence intensity of roGFP2-Orp1(Excitation wavelength is 488nm and the emission is followed at 515nm), which improved that the part can work properly.
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[[Image: T--BIT-China--iGEM2018-PartsOutput-TEF1experiment.png |center|400px|]]
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===Applications of BBa_K2765051===
 
===Applications of BBa_K2765051===

Latest revision as of 19:58, 17 October 2018


We ligated the promoter to roGFP2-Orp1 by Overlap Extension PCR and ligated it to the plasmid pESC-Trp containing the terminator cyc1 by enzyme digestion.

T--BIT-China--iGEM2018-PartsOutput-TEF1ligated.png

After sequencing verification, we transferred the correct plasmid into yeast for expression. Then we measured the fluorescence intensity of roGFP2-Orp1(Excitation wavelength is 488nm and the emission is followed at 515nm), which improved that the part can work properly.

T--BIT-China--iGEM2018-PartsOutput-TEF1experiment.png


Applications of BBa_K2765051

User Reviews

UNIQ4c9749c8333774db-partinfo-00000000-QINU UNIQ4c9749c8333774db-partinfo-00000001-QINU