Difference between revisions of "Part:BBa K2717006:Experience"

 
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===Applications of BBa_K2717006===
 
===Applications of BBa_K2717006===
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According to the western blot analysis, the glucose dehydrogenase has been well expressed (Fig. 1a). We inoculated the successfully expressed strains in the anti-antibiotic medium, induced by IPTG, and periodically measured the OD600 value of the bacterial liquid to draw a growth curve(Fig.1b and 1c)The results confirmed that gdh increased the environmental capacity of the cells and promoted the growth of the cells. The specific experiments and data processing can be found in our wiki.
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(a)                               (b)                            (c)                                   
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Fig.1 (a).the western blot result of gdh; (b)(c)the growth curve of bacteria.
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According to the method obtained in the literature, we determined the plasmid copy number in the bacteria at different time to verify the plasmid retention function of gdh. The experimental results confirmed that gdh has a small plasmid retention ability, but it did not achieve the expected effect, so we designed BBa_K2717010, BBa_K2717011 and BBa_K2717012 further detect the plasmid-retention capacity of gdh. The specific experiments and data processing can be found in our wiki.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 19:58, 17 October 2018


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2717006

According to the western blot analysis, the glucose dehydrogenase has been well expressed (Fig. 1a). We inoculated the successfully expressed strains in the anti-antibiotic medium, induced by IPTG, and periodically measured the OD600 value of the bacterial liquid to draw a growth curve(Fig.1b and 1c)The results confirmed that gdh increased the environmental capacity of the cells and promoted the growth of the cells. The specific experiments and data processing can be found in our wiki.


(a) (b) (c)

Fig.1 (a).the western blot result of gdh; (b)(c)the growth curve of bacteria.


According to the method obtained in the literature, we determined the plasmid copy number in the bacteria at different time to verify the plasmid retention function of gdh. The experimental results confirmed that gdh has a small plasmid retention ability, but it did not achieve the expected effect, so we designed BBa_K2717010, BBa_K2717011 and BBa_K2717012 further detect the plasmid-retention capacity of gdh. The specific experiments and data processing can be found in our wiki.

User Reviews

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