Difference between revisions of "Part:BBa K2717006"

Latest revision as of 19:55, 17 October 2018

GDH-v5 tag-his tag

This part is a coding one, in which Promoter lacI control the glucose dehydrogenase and V5 tag and His tag. Tag can be used to purify the proteins and to detect the expression of proteins. We constructed this vector to verify the growth-promoting effect of glucose dehydrogenase and its ability to retain plasmids.

function： We designed this part to purify the glucose dehydrogenase protein with the help of the V5 tag and His tag and detect its expression. After confirming the expression of gdh, we used the same strain, and under the induction of IPTG, quantitatively detected the growth of the cells and the retention of the plasmid to verify the growth-promoting effect and plasmid retention of glucose dehydrogenase.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1542
Illegal AgeI site found at 2434
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2416

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2717006 short</partinfo>
-GDH-v5 tag-his tag
+This part is a coding one, in which Promoter lacI control the glucose dehydrogenase and V5 tag and His tag. Tag can be used to purify the proteins and to detect the expression of proteins. We constructed this vector to verify the growth-promoting effect of glucose dehydrogenase and its ability to retain plasmids.
+function：
+We designed this part to purify the glucose dehydrogenase protein with the help of the V5 tag and His tag and detect its expression. After confirming the expression of gdh, we used the same strain, and under the induction of IPTG, quantitatively detected the growth of the cells and the retention of the plasmid to verify the growth-promoting effect and plasmid retention of glucose dehydrogenase.
 <!-- Add more about the biology of this part here