Difference between revisions of "Part:BBa K2656019"
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<partinfo>BBa_K2656019 short</partinfo>
 
<partinfo>BBa_K2656019 short</partinfo>

Revision as of 19:49, 17 October 2018

LuxI Coding Sequence


Part BBa_K2656013 is the Acyl-homoserine-lactone synthase (LuxI) coding sequence BBa_C0161 adapted to the Golden Braid grammar and so used with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/GB3 Golden Gate] assembly methods. It was codon optimized for E. coli.

LuxI coding sequence codifies for the 3OC6HSL (AHL) synthetase, so two signaling molecules of AHL interacts with two luxR proteins creating a complex which binds to the pLux promoter.

This part can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units in a one-step reaction with the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]