Difference between revisions of "Part:BBa K2765050"

 
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<partinfo>BBa_K2765050 short</partinfo>
 
<partinfo>BBa_K2765050 short</partinfo>
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===Introduction===
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This part consists of the promoter PTEF2 , redox sensor roGFP2-Orp1 and the terminator cyc1. The promoter PTEF2 is an constitutive promoter from Saccharomyces cerevisiae Cen.PK2-1C（see the details in part：BBa_K124001）. roGFP2-Orp1 is a fusion protein which can response to the redox status in the cell rapidly as well as sensitively(see the details in part:BBa_K2765043).
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[[Image: T--BIT-China--iGEM2018-PartsOutput-TEF2ligated.png |center|400px|]]
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This part is the expression cassette of the roGFP2-orp1 .We completed roGFP2-orp1 expression cassette started by the promoter TEF1.
 
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It can express green fluorescent protein roGFP2-orp1&#65292;and this protein can reflect the accumulation of ROS in cells.
 
  
 
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Revision as of 19:42, 17 October 2018


Complete roGFP2-Orp1 expression cassette started by the promoter TEF2

Introduction

This part consists of the promoter PTEF2 , redox sensor roGFP2-Orp1 and the terminator cyc1. The promoter PTEF2 is an constitutive promoter from Saccharomyces cerevisiae Cen.PK2-1C（see the details in part：BBa_K124001）. roGFP2-Orp1 is a fusion protein which can response to the redox status in the cell rapidly as well as sensitively(see the details in part:BBa_K2765043).

T--BIT-China--iGEM2018-PartsOutput-TEF2ligated.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1252
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1564