Difference between revisions of "Part:BBa K2623007"

Revision as of 19:39, 17 October 2018

SAHS coding region

Summary

Secreted heat soluble protein acting as a molecular shield in water-deficient condition. Tardigrade-specific intrinsically disordered proteins (TDPs) are essential for desiccation tolerance by forming non-crystalline amorphous solids upon desiccation, and this vitrified state mirrors their protective capabilities. The secretory TDP protein of water bear is used to improve the resistance of the bacteria under the conditions such as dehydration.

A separate fragment is 510bp

.
We transformed the plasmid to the E.coli(BL21), which is always used to express proteins with high efficience to verify the SAHS protein was produced successfully with a small scale.Besides, whether the SAHS protein with a signal peptide could be secreted was determined by SDS-PAGE.

Fig.1 The marker is on the lest, followed by our control group ( the BL21 with the empty plasmid), and the third well is the concentrated supernatant. The unit of the marker is "Kd".

More information about our project can be found on our results page.http://2018.igem.org/Team:XMU-China/Results

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 218
Illegal XhoI site found at 442
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 497
Illegal SapI.rc site found at 492

@@ Line 8: / Line 8: @@
 <br>
 We transformed the plasmid to the E.coli(BL21), which is always used to express proteins with high efficience to verify the SAHS protein was produced successfully with a small scale.Besides, whether the SAHS protein with a signal peptide could be secreted was determined by SDS-PAGE.
-<table><tr><th>[[Image:SAHS pro Gel 1.png|thumb|800px|Fig.1 The marker is on the lest, followed by our control group ( the BL21 with the empty plasmid), and the third well is the concentrated supernatant.]]</th><th></table><br>
+<table><tr><th>[[Image:SAHS pro Gel 1.png|thumb|800px|Fig.1 The marker is on the lest, followed by our control group ( the BL21 with the empty plasmid), and the third well is the concentrated supernatant. The unit of the marker is "Kd". ]]</th><th></table><br>
 More information about our project can be found on our results page.http://2018.igem.org/Team:XMU-China/Results <br>