Difference between revisions of "Part:BBa K2765053:Experience"
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We ligated the promoter to roGFP2-Orp1 by Overlap Extension PCR and ligated it to the plasmid pESC-Trp containing the terminator cyc1 by enzyme digestion. | We ligated the promoter to roGFP2-Orp1 by Overlap Extension PCR and ligated it to the plasmid pESC-Trp containing the terminator cyc1 by enzyme digestion. | ||
| − | [[Image: T--BIT-China--iGEM2018-PartsOutput-FBA1 ligated.png |center| | + | [[Image: T--BIT-China--iGEM2018-PartsOutput-FBA1 ligated.png |center|300px|]] |
After sequencing verification, we transferred the correct plasmid into yeast for expression. Then we measured the fluorescence intensity of roGFP2-Orp1(Excitation wavelength is 488nm and the emission is followed at 515nm), which improved that the part can work properly. | After sequencing verification, we transferred the correct plasmid into yeast for expression. Then we measured the fluorescence intensity of roGFP2-Orp1(Excitation wavelength is 488nm and the emission is followed at 515nm), which improved that the part can work properly. | ||
| − | [[Image: T--BIT-China--iGEM2018-PartsOutput-FBA1exprimentresult.png |center| | + | [[Image: T--BIT-China--iGEM2018-PartsOutput-FBA1exprimentresult.png |center|400px|]] |
Latest revision as of 19:39, 17 October 2018
We ligated the promoter to roGFP2-Orp1 by Overlap Extension PCR and ligated it to the plasmid pESC-Trp containing the terminator cyc1 by enzyme digestion.
After sequencing verification, we transferred the correct plasmid into yeast for expression. Then we measured the fluorescence intensity of roGFP2-Orp1(Excitation wavelength is 488nm and the emission is followed at 515nm), which improved that the part can work properly.
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UNIQc80dfe185b8abe98-partinfo-00000000-QINU UNIQc80dfe185b8abe98-partinfo-00000001-QINU