Difference between revisions of "Part:BBa K2765053:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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how you used this part and how it worked out.
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We ligated the promoter to roGFP2-Orp1 by Overlap Extension PCR and ligated it to the plasmid pESC-Trp containing the terminator cyc1 by enzyme digestion.
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[[Image: T--BIT-China--iGEM2018-PartsOutput-FBA1 ligated.png |center|400px|]]
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After sequencing verification, we transferred the correct plasmid into yeast for expression. Then we measured the fluorescence intensity of roGFP2-Orp1(Excitation wavelength is 488nm and the emission is followed at 515nm), which improved that the part can work properly.
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[[Image: T--BIT-China--iGEM2018-PartsOutput-FBA1exprimentresult.png |center|600px|]]
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===Applications of BBa_K2765053===
 
===Applications of BBa_K2765053===

Revision as of 19:38, 17 October 2018


We ligated the promoter to roGFP2-Orp1 by Overlap Extension PCR and ligated it to the plasmid pESC-Trp containing the terminator cyc1 by enzyme digestion.

T--BIT-China--iGEM2018-PartsOutput-FBA1 ligated.png

After sequencing verification, we transferred the correct plasmid into yeast for expression. Then we measured the fluorescence intensity of roGFP2-Orp1(Excitation wavelength is 488nm and the emission is followed at 515nm), which improved that the part can work properly.

T--BIT-China--iGEM2018-PartsOutput-FBA1exprimentresult.png


Applications of BBa_K2765053

User Reviews

UNIQ02869fade81965e7-partinfo-00000000-QINU UNIQ02869fade81965e7-partinfo-00000001-QINU