Difference between revisions of "Part:BBa K2550001:Design"
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<partinfo>BBa_K2550001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2550001 SequenceAndFeatures</partinfo> | ||
− | + | The RNA sequence was obtained from the Georgia Institue of Technology that was number 2 of the 144 generation orthogonal toehold switch collection from the Collins Paper titled, Toehold Switches: De-Novo-Designed Regulators of Gene Expression. | |
===Design Notes=== | ===Design Notes=== |
Revision as of 19:23, 17 October 2018
T7 promoter with a trigger sequence to match the toehold sequence of part BBa_K2550000
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 104
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
The RNA sequence was obtained from the Georgia Institue of Technology that was number 2 of the 144 generation orthogonal toehold switch collection from the Collins Paper titled, Toehold Switches: De-Novo-Designed Regulators of Gene Expression.
Design Notes
This is meant to be used with toehold part. Needs to be used in BL21 cells that have a T7 polymerase.
Source
Collins Paper