Difference between revisions of "Part:BBa K2717005"

 
 
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<partinfo>BBa_K2717005 short</partinfo>
 
<partinfo>BBa_K2717005 short</partinfo>
  
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mprA-GAPGH promoter-emrr binding promoter-mCherry
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he vector is linked with GAPDH promoter, emrR ,sddsdsaaadd emrR binding promoter and mcherry fluorescent protein, which can realize the response of mcherry fluorescence intensity to the concentration of salicylic acid in the outside. After the strain is expanded and cultured, different concentrations of salicylic acid are added for induction. It can be found that the higher the concentration of salicylic acid, the greater the overall fluorescence intensity.
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Function: It can realize the fluorescence intensity of mcherry fluorescent egg in response to salicylic acid concentration.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 19:20, 17 October 2018


mprA-GAPGH promoter-emrr binding promoter-mCherry

he vector is linked with GAPDH promoter, emrR ,sddsdsaaadd emrR binding promoter and mcherry fluorescent protein, which can realize the response of mcherry fluorescence intensity to the concentration of salicylic acid in the outside. After the strain is expanded and cultured, different concentrations of salicylic acid are added for induction. It can be found that the higher the concentration of salicylic acid, the greater the overall fluorescence intensity.

Function: It can realize the fluorescence intensity of mcherry fluorescent egg in response to salicylic acid concentration.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 76
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2037
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 687