Difference between revisions of "Part:BBa K2627000:Design"
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===Design notes=== removing two PAS domains by Gibson Assembly in Freiburg BioBrick assembly standard.
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===Source=== CcaS originated in Synechocystis PCC 6803
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__NOTOC__
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===Reference=== Nakajima, M., et al., Construction of a Miniaturized Chromatic Acclimation Sensor from Cyanobacteria with Reversed Response to a Light Signal. Scientific Reports, 2016. 6(1).
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<partinfo>BBa_K1172501 short</partinfo>
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<partinfo>BBa_K1172501 SequenceAndFeatures</partinfo>
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===Design notes===  
 +
removing two PAS domains by Gibson Assembly in Freiburg BioBrick assembly standard.
 +
===Source===  
 +
CcaS originated in Synechocystis PCC 6803
 +
===Reference===  
 +
Nakajima, M., et al., Construction of a Miniaturized Chromatic Acclimation Sensor from Cyanobacteria with Reversed Response to a Light Signal. Scientific Reports, 2016. 6(1).

Revision as of 19:16, 17 October 2018


Outer membrane porin OprF from Pseudomonas fluorescens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 764
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1156
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 677

Design notes

removing two PAS domains by Gibson Assembly in Freiburg BioBrick assembly standard.

Source

CcaS originated in Synechocystis PCC 6803

Reference

Nakajima, M., et al., Construction of a Miniaturized Chromatic Acclimation Sensor from Cyanobacteria with Reversed Response to a Light Signal. Scientific Reports, 2016. 6(1).