Difference between revisions of "Part:BBa K592001"

Revision as of 19:13, 17 October 2018

CcaS, green light sensor from Synechocystis sp. PCC6803

CcaS is a green light-induced sensor protein from the genome of Synechocystis sp. PCC6803. It requires PCB as the chromophore to function. PCB is made by ho1 and PcyA. Furthermore, CcaS works in conjunction with CcaR, its response regulator. This is the green light sensor used by Tabor in his multichromatic light-sensing system which composed of CcaS/CcaR and Cph8/EnvZ.

A new part (BBa_K2627000) constructed by SDU-China was improved from CcaS by deleting about 800 bases corresponding to two PAS domains since we were inspired by Professor Koji’s work. Part BBa_K2627000 (CcaS#4) is a varient of CcaS having an opposite response to green/red light. High intensity of fluorescence can be seen when bacteria are cultured under red light while fluorescence is pretty weak under green light.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 606
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 2252
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1270
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K592001 short</partinfo>
-CcaS is a green light-induced sensor protein from the genome of Synechocystis sp. PCC6803. It requires PCB as the chromophore to function. PCB is made by [[Part:BBa_I15008|ho1]] and [[Part:BBa_I15009|PcyA]]. Furthermore, CcaS works in conjunction with [[Part:BBa_K592002|CcaR]], its response regulator. This is the green light sensor used by Tabor in his multichromatic light-sensing system which composed of CcaS/CcaR and Cph8/EnvZ.
+CcaS is a green light-induced sensor protein from the genome of Synechocystis sp. PCC6803. It requires PCB as the chromophore to function. PCB is made by [[Part:BBa_I15008|ho1]] and [[Part:BBa_I15009|PcyA]]. Furthermore, CcaS works in conjunction with [[Part:BBa_K592002|CcaR]], its response regulator. This is the green light sensor used by Tabor in his multichromatic light-sensing system which composed of CcaS/CcaR and Cph8/EnvZ. <br>
+<p>A new part (<partinfo>BBa_K2627000</partinfo>) constructed by SDU-China was improved from CcaS by deleting about 800 bases corresponding to two PAS domains since we were inspired by Professor Koji’s work. Part BBa_K2627000 (CcaS#4) is a varient of CcaS having an opposite response to green/red light. High intensity of fluorescence can be seen when bacteria are cultured under red light while fluorescence is pretty weak under green light.</p>
 <!-- Add more about the biology of this part here