Difference between revisions of "Part:BBa K2717023:Experience"

Revision as of 19:09, 17 October 2018

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2717023

We used In-Fusion Cloning tech to link our composite to pUCyder. The whole pathway can be described in Figure 1.

Figure 1 the constuction of part BBa_K2717018

Then the plasmid is transfered into E.coli BL21. We use Flow Cytometer (FCM) to mearsure the bacteria's number and a relative fluorescence. Figure 2 is one of the FCM result graphs. The specific experiments and data processing methods can be found in our wiki. Finally, by comparing the final relative fluorescent intensity with that of control group, we found the exogenous CI sequence causes a 150% improvement to pink fluorescent intensity. That is to say, CI protein can indeed increase the expression of following gene.

Table 1 the comparison of GFP expression between CI-activation and control.

User Reviews

UNIQ378bb70a8b2e3700-partinfo-00000000-QINU UNIQ378bb70a8b2e3700-partinfo-00000001-QINU

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 ===Applications of BBa_K2717023===
+We used In-Fusion Cloning tech to link our composite to pUCyder. The whole pathway can be described in Figure 1.
+Figure 1 the constuction of part BBa_K2717018
+Then the plasmid is transfered into E.coli BL21. We use Flow Cytometer (FCM) to mearsure the bacteria's number and a relative fluorescence. Figure 2 is one of the FCM result graphs. The specific experiments and data processing methods can be found in our wiki. Finally, by comparing the final relative fluorescent intensity with that of control group, we found the exogenous CI sequence causes a 150% improvement to pink fluorescent intensity. That is to say, CI protein can indeed increase the expression of following gene.
+Table 1 the comparison of GFP expression between CI-activation and control.
 ===User Reviews===