Difference between revisions of "Part:BBa K2533000"
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ldhD can produce lactate, and lldP can release it from cytoplasm to the environment.
 
ldhD can produce lactate, and lldP can release it from cytoplasm to the environment.
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<h1>'''Characterization'''</h1>
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This is a two section for lactate produce and transfer part.
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[[File:T--HUST-China--2018-tonglu-ldhD-lldP.png ‎|400px|thumb|center|Figure1:ldhD-lldP]]
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<h2>DNA Gel Electrophoretic</h2>
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After we link ldhD and lldP successfully, we design several tags and then add these tags through PCR with an intention to confirm the expression of ldhD and lldP. As the figure shows, we get the genetic stripe about 3000bp which means the PCR is successful.
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[[File:T--HUST-China--2018-jiaotu-ldhD-ldhDC.jpeg|400px|thumb|center|Figure2:Expression of ldhD]]
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<h2>Metabolic figure</h2>
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[[File:T--HUST-China--2018-metabolic figure1.png|400px|thumb|center|Figure3:Metabolic figure of cyanobacteria with ldhD-lldP]]
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As the figure shows, with the help of light and PSI, Synechocystis sp. PCC 6803(cyanobacteria) with ldhD-lldP absorb CO2 into Calvin cycle and produce pyruvate. And then ldhD can transfer pyruvate into lactate which will be transported to the environment by lldP.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 19:07, 17 October 2018


RBS-ldhD-RBS-lldP

ldhD can produce lactate, and lldP can release it from cytoplasm to the environment.

Characterization

This is a two section for lactate produce and transfer part.

Figure1:ldhD-lldP

DNA Gel Electrophoretic

After we link ldhD and lldP successfully, we design several tags and then add these tags through PCR with an intention to confirm the expression of ldhD and lldP. As the figure shows, we get the genetic stripe about 3000bp which means the PCR is successful.

Figure2:Expression of ldhD

Metabolic figure

Figure3:Metabolic figure of cyanobacteria with ldhD-lldP

As the figure shows, with the help of light and PSI, Synechocystis sp. PCC 6803(cyanobacteria) with ldhD-lldP absorb CO2 into Calvin cycle and produce pyruvate. And then ldhD can transfer pyruvate into lactate which will be transported to the environment by lldP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 780
    Illegal BglII site found at 1325
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1461
    Illegal AgeI site found at 1924
    Illegal AgeI site found at 2362
    Illegal AgeI site found at 2521
    Illegal AgeI site found at 2533
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1035