Difference between revisions of "Part:BBa K2717015:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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T7-TGATG
 
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 +
In the course of the experiment, GFP, T7 RNA Polymerase and gdh are obtained by PCR, then GFP is ligated with T7 RNA Polymerase by overlap method, and then our modified plasmid pUCyder is linearized by single restriction enzyme, through infusion. The way to connect the pieces together.
  
 
===Source===
 
===Source===

Revision as of 19:02, 17 October 2018


GFP-TGATG-T7 RNA Polymerase-GDH-v5 tag-his tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3389
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 5252
    Illegal AgeI site found at 6144
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6126
    Illegal BsaI.rc site found at 644


Design Notes

In the course of the experiment, GFP, T7 RNA Polymerase and gdh are obtained by PCR, then GFP is ligated with T7 RNA Polymerase by overlap method, and then our modified plasmid pUCyder is linearized by single restriction enzyme, through infusion. The way to connect the pieces together.

Source

T7-TGATG

References