Difference between revisions of "Part:BBa K2569031"

Revision as of 19:00, 17 October 2018

PdnaA -NicA2

Expressing this BioBrick in Pseudomonas fluorescence-pf5 cells enables the bacterium to degrade nicotine. NicA2 is a monomer with a size of 53 kDa t, catalyzing the key first step in nicotine catalytic metabolism. Heterologously overexpressing NicA2 in P.fluorescence-pf5 can convert nicotine to pseudo-oxidation can be achieved by resting cells of P.fluorescence-pf5 as a catalyst in a whole-cell reaction.So, with our strong promoper PdnaA enhancing, NicA2 is overexpressed and survived in high concentration of nicotin. Nicotin can get fully degradated.

Nicotine Degradation Pathway

  
  Fig. Nicotine degradation pathway

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1393
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2569031 short</partinfo>
-Expressing this BioBrick in <i>Pseudomonas fluorescence</i>-pf5 cells enables the bacterium to degrade nicotine. NicA2 is a monomer with a size of 53 kDa t, catalyzing the key first step in nicotine catalytic metabolism. Heterologously overexpressing NicA2 in <i>P.fluorescence</i>-pf5 can convert nicotine to pseudo-oxidation can be achieved by resting cells of <i>P.fluorescence</i>-pf5 as a catalyst in a whole-cell reaction.
+Expressing this BioBrick in <i>Pseudomonas fluorescence</i>-pf5 cells enables the bacterium to degrade nicotine. NicA2 is a monomer with a size of 53 kDa t, catalyzing the key first step in nicotine catalytic metabolism. Heterologously overexpressing NicA2 in <i>P.fluorescence</i>-pf5 can convert nicotine to pseudo-oxidation can be achieved by resting cells of <i>P.fluorescence</i>-pf5 as a catalyst in a whole-cell reaction.So, with our strong promoper PdnaA enhancing, NicA2 is overexpressed and survived in high concentration of nicotin. Nicotin can get fully degradated.
 ===Nicotine Degradation Pathway===