Difference between revisions of "Part:BBa K2847002"
 
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<b>NB! This part is NOT BioBrick compatible!</b> It is designed to be used as a PCR matrix for the amplification of the pSC101* thermosensitive origin of replication. The cheapest and fastest way to put it in a pSB1-based biobrick vector is to use Aqua cloning - see the Main page of part <partinfo>BBa_K2515002</partinfo> for more details.
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<b>NB! This part is NOT BioBrick compatible! It contains illegal SpeI site!</b> It is designed to be used as a PCR matrix for the amplification of the pSC101* thermosensitive origin of replication. The cheapest and fastest way to put it in a pSB1-based biobrick vector is to use Aqua cloning - see the Main page of part <partinfo>BBa_K2515002</partinfo> for more details.
  
 
   
 
   

Latest revision as of 18:54, 17 October 2018


pSC101 ori + RepA (thermosensitive mutant) + AmpR gene

pSC101 ori + RepA (thermosensitive mutant) + AmpR gene amplified from vector pE-FLP (Addgene Plasmid #45978)


NB! This part is NOT BioBrick compatible! It contains illegal SpeI site! It is designed to be used as a PCR matrix for the amplification of the pSC101* thermosensitive origin of replication. The cheapest and fastest way to put it in a pSB1-based biobrick vector is to use Aqua cloning - see the Main page of part BBa_K2515002 for more details.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 1163
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 1163
    Illegal NotI site found at 1482
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 1163
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 1163
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2128