Difference between revisions of "Part:BBa K2717008:Design"

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===Design Notes===
 
===Design Notes===
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In this composited part, we link GFP with mCherry by using sequence "TGATG" and the whole coding is controlled by Promoter lacI and RBS B0034. We design this part to test the efficiency of start-stop codon "TGATG".
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We adjusted the codon of the last lysine of GFP from AAA to AAG.
  
 
===Source===
 
===Source===

Latest revision as of 18:44, 17 October 2018


GFP-TGATG-mCherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1425
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


Design Notes

We adjusted the codon of the last lysine of GFP from AAA to AAG.

Source

GFP and mCherry is stored in our own lab.

References