Difference between revisions of "Part:BBa K2569031"
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Expressing this BioBrick in <i>E. coli</i> cells enables the bacterium to degrade nicotine. NicA2 is a monomer with a size of 53 kDa t, catalyzing the key first step in nicotine catalytic metabolism. Heterologously overexpressing NicA2 in <i>E. coli</i> can convert nicotine to pseudo-oxidation can be achieved by resting cells of E. coli as a catalyst in a whole-cell reaction. | Expressing this BioBrick in <i>E. coli</i> cells enables the bacterium to degrade nicotine. NicA2 is a monomer with a size of 53 kDa t, catalyzing the key first step in nicotine catalytic metabolism. Heterologously overexpressing NicA2 in <i>E. coli</i> can convert nicotine to pseudo-oxidation can be achieved by resting cells of E. coli as a catalyst in a whole-cell reaction. | ||
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<img src="https://static.igem.org/mediawiki/2018/7/70/T--SKLMT-China--nicotine-pathway.png"alt="Nicotine degradation pathway" /> | <img src="https://static.igem.org/mediawiki/2018/7/70/T--SKLMT-China--nicotine-pathway.png"alt="Nicotine degradation pathway" /> | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 18:42, 17 October 2018
PdnaA -NicA2
Expressing this BioBrick in E. coli cells enables the bacterium to degrade nicotine. NicA2 is a monomer with a size of 53 kDa t, catalyzing the key first step in nicotine catalytic metabolism. Heterologously overexpressing NicA2 in E. coli can convert nicotine to pseudo-oxidation can be achieved by resting cells of E. coli as a catalyst in a whole-cell reaction.
<img src="
"alt="Nicotine degradation pathway" />
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1393
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]