Difference between revisions of "Part:BBa K2569031"

 
Line 3: Line 3:
 
<partinfo>BBa_K2569031 short</partinfo>
 
<partinfo>BBa_K2569031 short</partinfo>
  
−
Expressing this BioBrick in E. coli enables the bacterium to degrade nicotine. Nicotine is one of the most harmful ingredients in cigarette and is the biggest resistance to smoking cessation. NicA2 is a monomer with a size of 53 kDa t, catalyzing the key first step in nicotine catalytic metabolism. Heterologously overexpressing NicA2 in E. coli can convert nicotine to pseudo-oxidation can be achieved by resting cells of E. coli as a catalyst in a whole-cell reaction.
+
Expressing this BioBrick in <i>E. coli</i> cells enables the bacterium to degrade nicotine. NicA2 is a monomer with a size of 53 kDa t, catalyzing the key first step in nicotine catalytic metabolism. Heterologously overexpressing NicA2 in <i>E. coli</i> can convert nicotine to pseudo-oxidation can be achieved by resting cells of E. coli as a catalyst in a whole-cell reaction.
  
−
<!-- Add more about the biology of this part here
+
<!-- Add more about the biology of this part here>
 +
<img src="https://static.igem.org/mediawiki/2018/7/70/T--SKLMT-China--nicotine-pathway.png"alt="Nicotine degradation pathway" />
 
===Usage and Biology===
 
===Usage and Biology===
  

Revision as of 18:40, 17 October 2018


PdnaA -NicA2

Expressing this BioBrick in E. coli cells enables the bacterium to degrade nicotine. NicA2 is a monomer with a size of 53 kDa t, catalyzing the key first step in nicotine catalytic metabolism. Heterologously overexpressing NicA2 in E. coli can convert nicotine to pseudo-oxidation can be achieved by resting cells of E. coli as a catalyst in a whole-cell reaction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1393
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]