Difference between revisions of "Part:BBa K2685006"
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<partinfo>BBa_K2685006 short</partinfo> | <partinfo>BBa_K2685006 short</partinfo> | ||
| − | + | This part expresses an efflux pump protein that selectively pumps tetracycline from the cell with Mg + isomeric tetracycline to restrict tetracycline entry into the cell ribosome, thereby enhancing the tolerance of E. coli to tetracycline by inserting E. coli containing the psb1c3 empty plasmid and our E. coli with this part in the absence of The ABS value was measured under the same concentration of tetracycline. It can be seen that E. coli containing only psb1c3 empty plasmid has little tolerance to tetracycline, and we added this part E.coli has certain tolerance to tetracycline. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 18:21, 17 October 2018
TetO+TetA
This part expresses an efflux pump protein that selectively pumps tetracycline from the cell with Mg + isomeric tetracycline to restrict tetracycline entry into the cell ribosome, thereby enhancing the tolerance of E. coli to tetracycline by inserting E. coli containing the psb1c3 empty plasmid and our E. coli with this part in the absence of The ABS value was measured under the same concentration of tetracycline. It can be seen that E. coli containing only psb1c3 empty plasmid has little tolerance to tetracycline, and we added this part E.coli has certain tolerance to tetracycline.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1043
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 897
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 343
Illegal NgoMIV site found at 503
Illegal NgoMIV site found at 871 - 1000COMPATIBLE WITH RFC[1000]