Difference between revisions of "Part:BBa K2557037"

 
 
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Enhanced Green Fluorescent Protein is a fluorescent protein and is a widely used reporter gene in synthetic biology.
 
Enhanced Green Fluorescent Protein is a fluorescent protein and is a widely used reporter gene in synthetic biology.
  
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===Usage and Biology===
 
  
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===Characterization===
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Fig. 1 Fluorescence microscope observation of HEK 293T only transfect with plasmids containing promoters with tetO sequence.
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Fig. 1 shows that EGFP can be normally expressed in HEK 293T, and the fluorescence intensity depends on the amount of expression.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2557037 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2557037 SequenceAndFeatures</partinfo>

Latest revision as of 18:07, 17 October 2018


EGFP

Enhanced Green Fluorescent Protein is a fluorescent protein and is a widely used reporter gene in synthetic biology.


Characterization

Fig. 1 Fluorescence microscope observation of HEK 293T only transfect with plasmids containing promoters with tetO sequence.

Fig. 1 shows that EGFP can be normally expressed in HEK 293T, and the fluorescence intensity depends on the amount of expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 6
  • 1000
    COMPATIBLE WITH RFC[1000]