Difference between revisions of "Part:BBa K2557038"
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TetO is a DNA sequence that can be bound by the TetR protein, which in turn prevents the expression of downstream genes. | TetO is a DNA sequence that can be bound by the TetR protein, which in turn prevents the expression of downstream genes. | ||
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| + | <img src="https://static.igem.org/mediawiki/parts/d/db/T--NAU-CHINA-liuzhang_lvse.jpg"width="600"/> | ||
| + | </html> | ||
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| + | Fig.1 Inhibition of tetR on promoter with tetO sequence | ||
| + | (A)Fluorescence microscope observation of HEK 293T only transfect with plasmids containing promoters with tetO sequence. | ||
| + | (B)Fluorescence microscope observation of HEK 293T transfect with plasmids containing promoters with tetO sequence and tetR. | ||
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| + | Fig. 1 shows that TetR has obvious inhibitory effects on the three promoters downstream of TetO. | ||
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| + | ===References=== | ||
| + | 1. Ramos, J. L. et al. The TetR Family of Transcriptional Repressors The TetR Family of Transcriptional Repressors. Microbiol. Mol. Biol. Rev. 69, 326–356 (2005). | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K2557038 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2557038 SequenceAndFeatures</partinfo> | ||
Latest revision as of 18:04, 17 October 2018
TetO
TetO is a DNA sequence that can be bound by the TetR protein, which in turn prevents the expression of downstream genes.
Characterization
Fig.1 Inhibition of tetR on promoter with tetO sequence (A)Fluorescence microscope observation of HEK 293T only transfect with plasmids containing promoters with tetO sequence. (B)Fluorescence microscope observation of HEK 293T transfect with plasmids containing promoters with tetO sequence and tetR.
Fig. 1 shows that TetR has obvious inhibitory effects on the three promoters downstream of TetO.
References
1. Ramos, J. L. et al. The TetR Family of Transcriptional Repressors The TetR Family of Transcriptional Repressors. Microbiol. Mol. Biol. Rev. 69, 326–356 (2005).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]