Difference between revisions of "Part:BBa K2570021:Design"

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===Design Notes===
 
===Design Notes===
 +
This is an improved version of [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2120002 BBa_K2120002].
 +
 
dsrA is a temperature control promoter. The temperature range of dsrA is from 25 degree to 42 degree. With temperature increase, the promote efficiency is getting higher.
 
dsrA is a temperature control promoter. The temperature range of dsrA is from 25 degree to 42 degree. With temperature increase, the promote efficiency is getting higher.
  

Revision as of 18:00, 17 October 2018


dsrA+B0034+GFP+B0032+mazF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 721


Design Notes

This is an improved version of BBa_K2120002.

dsrA is a temperature control promoter. The temperature range of dsrA is from 25 degree to 42 degree. With temperature increase, the promote efficiency is getting higher.

We obtained the wild-type mazf toxin protein sequence from E. coli and conducted experiments as a positive control.

Source

It comes from E.coli.


References

1] Repoila, F. and S. Gottesman (2003). "Temperature Sensing by the dsrA Promoter." Journal of Bacteriology 185(22): 6609-6614.