Difference between revisions of "Part:BBa K2587018"

Revision as of 17:35, 17 October 2018

P_rpa_RBS_E_T

This construct contains lysis gene E. E is a lysis gene from the bacteriophage PhiX174. This gene is used in our co-culture to induce self lysis of E. coli under the control of the pRpa promoter. This promoter is part of the Quorum sensing system of Rhodopseudomonas palustris. Together with the other components of the system, RpaR and RpaI, this promoter induces expression of the lysis gene.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 93

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 <partinfo>BBa_K2587018 short</partinfo>
-This construct contains lysis gene <i>E</i> is a lysis gene from the bacteriophage PhiX174. This gene is used in our co-culture to induce self lysis of <i>E. coli</i> under the control of the pRpa promoter. This promoter is part of the Quorum sensing system of <i>Rhodopseudomonas palustris</i>. Together with the other components of the system, RpaR and RpaI , this promoter will induce expression of the lysis gene.
+This construct contains lysis gene <i>E</I>. <i>E</I> is a lysis gene from the bacteriophage PhiX174. This gene is used in our co-culture to induce self lysis of <i>E. coli</i> under the control of the pRpa promoter. This promoter is part of the Quorum sensing system of <i>Rhodopseudomonas palustris</i>. Together with the other components of the system, RpaR and RpaI, this promoter induces expression of the lysis gene.
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