Difference between revisions of "Part:BBa K2569032"
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2569032 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2569032 SequenceAndFeatures</partinfo>
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==Improvement by replacing induced promoter with constitutive promoter==
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[http://2018.igem.org/Team:SKLMT-China Team SKLMT-China 2018] Improved the part(<partinfo>BBa_J04450</partinfo>)by replacing induced promoter with constitutive promoter <br>
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lacI is present on the genome of many host cells, which suggests that he normal expression of RFP requires the induction of IPTG and need 18 hours of waiting. We replaced the lac promoter in front of the RFP gene with the strong pf-5 promoter PampC. And hope that RFP, as a visible marker for the naked eye, can be expressed more pronouncedly and faster in in <latin>Pseudomonas fluorescens </latin>pf-5.
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Revision as of 17:31, 17 October 2018


PampC -mRFP

The mRFP behind constitutive promoter PampC, who is the strongest member in our promoter family, leading to enhanced expression of mRFP. lacI is present on the genome of many host cells, which suggests that he normal expression of RFP requires the induction of IPTG and need 18 hours of waiting. We replaced the lac promoter in front of the RFP gene with the strong pf-5 promoter PampC. And hope that RFP, as a visible marker for the naked eye, can be expressed more pronouncedly and faster in in <latin>Pseudomonas fluorescens </latin>pf-5.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 631
    Illegal AgeI site found at 743
  • 1000
    COMPATIBLE WITH RFC[1000]

Improvement by replacing induced promoter with constitutive promoter

[http://2018.igem.org/Team:SKLMT-China Team SKLMT-China 2018] Improved the part(BBa_J04450)by replacing induced promoter with constitutive promoter 

lacI is present on the genome of many host cells, which suggests that he normal expression of RFP requires the induction of IPTG and need 18 hours of waiting. We replaced the lac promoter in front of the RFP gene with the strong pf-5 promoter PampC. And hope that RFP, as a visible marker for the naked eye, can be expressed more pronouncedly and faster in in <latin>Pseudomonas fluorescens </latin>pf-5.