Difference between revisions of "Part:BBa K2587014"
Line 3: | Line 3: | ||
<partinfo>BBa_K2587014 short</partinfo> | <partinfo>BBa_K2587014 short</partinfo> | ||
− | LasR is a component of the Quorum sensing system and is used to start transciption of a target gene upon binding to the quorum sensing molecule AHL and the pLas promoter. The system is originally from <i>Pseudomonas aeruginosa</i> but | + | LasR is a component of the Quorum sensing system and is used to start transciption of a target gene upon binding to the quorum sensing molecule AHL and the pLas promoter. The system is originally from <i>Pseudomonas aeruginosa</i> but we codon optimized it for <i>E. coli</i>. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 17:31, 17 October 2018
P_RBS_lasR_T
LasR is a component of the Quorum sensing system and is used to start transciption of a target gene upon binding to the quorum sensing molecule AHL and the pLas promoter. The system is originally from Pseudomonas aeruginosa but we codon optimized it for E. coli.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 643
Illegal SpeI site found at 802 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 643
Illegal SpeI site found at 802 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 643
Illegal SpeI site found at 802 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 643
Illegal SpeI site found at 802 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 795