Difference between revisions of "Part:BBa K2587029"
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| − | <p>1. Schrumpf, Barbel, et al. "A functionally split pathway for lysine synthesis in <i>Corynebacterium glutamicium." Journal of bacteriology</i> 173.14 (1991): 4510-4516.:<br> | + | <p>1. Schrumpf, Barbel, et al. "A functionally split pathway for lysine synthesis in <i>Corynebacterium glutamicium.</i>"<i> Journal of bacteriology</i> 173.14 (1991): 4510-4516.:<br> |
https://jb.asm.org/content/173/14/4510.short</p> | https://jb.asm.org/content/173/14/4510.short</p> | ||
Revision as of 17:26, 17 October 2018
Pj23102_RBS_ddh_T
Transcription unit with the gene ddh coding for Meso-diaminopimelate D-dehydrogenase. The enzyme catalyses the reaction from L-2-amino-6-oxopimelate to D,L-2,6-diaminopimelate in the lysine biosynthesis pathway of Corynebacterium glutamicum1. We use it as a multi step enzyme for the lysine biosynthesis in Escherichia coli, since the wiltype normally needs five enzymes for the reaction from L-2-amino-6-oxopimelate to D,L-2,6-diaminopimelate.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 422
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 390
Illegal NgoMIV site found at 615
Illegal AgeI site found at 825 - 1000COMPATIBLE WITH RFC[1000]
1. Schrumpf, Barbel, et al. "A functionally split pathway for lysine synthesis in Corynebacterium glutamicium." Journal of bacteriology 173.14 (1991): 4510-4516.:
https://jb.asm.org/content/173/14/4510.short