Difference between revisions of "Part:BBa K2765026"
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This part consists of the promoter gal1, redox sensor yno1 and the terminator cyc1. The promoter gal1 is an constitutive promoter from Saccharomyces cerevisiae CENPK2-1C.yno1（yeast NADPH oxidase 1） is located in the endoplasmic reticulum (ER) and produces superoxide in a NADPH-dependent fashion encodes a genuine NADPH oxidase (NOX enzymes),(see the details in part:BBA-K2765007).And thus the target gene yno1 is induced by galactose and repressed by glucose.as shown in Fig.1.
 
This part consists of the promoter gal1, redox sensor yno1 and the terminator cyc1. The promoter gal1 is an constitutive promoter from Saccharomyces cerevisiae CENPK2-1C.yno1（yeast NADPH oxidase 1） is located in the endoplasmic reticulum (ER) and produces superoxide in a NADPH-dependent fashion encodes a genuine NADPH oxidase (NOX enzymes),(see the details in part:BBA-K2765007).And thus the target gene yno1 is induced by galactose and repressed by glucose.as shown in Fig.1.
  
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https://static.igem.org/mediawiki/2018/1/12/T--BIT-China--Parts_regulator_Fig.1_PGal1%2Byno1%2BTCYC1.png
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[[Image: T--BIT-China--iGEM2018-Partsregulator-Fig.1-geneline.png |center|400px|]]
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Revision as of 17:12, 17 October 2018


GAL1p+yno1+CYC1t


This part consists of the promoter gal1, redox sensor yno1 and the terminator cyc1. The promoter gal1 is an constitutive promoter from Saccharomyces cerevisiae CENPK2-1C.yno1（yeast NADPH oxidase 1） is located in the endoplasmic reticulum (ER) and produces superoxide in a NADPH-dependent fashion encodes a genuine NADPH oxidase (NOX enzymes),(see the details in part:BBA-K2765007).And thus the target gene yno1 is induced by galactose and repressed by glucose.as shown in Fig.1.

T--BIT-China--iGEM2018-Partsregulator-Fig.1-geneline.png





Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2452
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 669
    Illegal XhoI site found at 2430
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 286
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2281