Difference between revisions of "Part:BBa K2719007:Experience"

Revision as of 17:06, 17 October 2018

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2719007

This part was cloned on pSB1C3 using EcoRI and PstI, later it was transformed on E.coli DH5a (Figure 1).

Figure 1. Colonies transformed with Collagen V-like expression device, the red strain indicates a successful clonation and transformation of the plasmid

Then the plasmid was extracted and run on an agarose gel electrophoresis (Figure 2). Later an restriction was performed using NotI and was run on an agarose gel (Figure 3) to probe the presence of the insert. Later the extracted plasmid was transformed on E.coli Rossetta (DE3).

User Reviews

UNIQ4bb095eb25c497b3-partinfo-00000000-QINU UNIQ4bb095eb25c497b3-partinfo-00000001-QINU

@@ Line 6: / Line 6: @@
 ===Applications of BBa_K2719007===
 <p>This part was cloned on pSB1C3 using EcoRI and PstI, later it was transformed on <i>E.coli</i> DH5a (Figure 1).</p>
+[[file:T--TecCEM--BBCol5Colonies.png|500px]]
+<p><i>Figure 1.</i> Colonies transformed with Collagen V-like expression device, the red strain indicates a successful clonation and transformation of the plasmid</p>
-<p>Then the plasmid was extracted and run on an agarose gel electrophoresis (Figure 2).  Later an restriction was performed using NotI and was run on an agarose gel (Figure 3) to probe the presence of the insert. Later the extracted plasmid was transformed on <i>E.coli</i> Rossetto (DE3).</p>
+<p>Then the plasmid was extracted and run on an agarose gel electrophoresis (Figure 2).  Later an restriction was performed using NotI and was run on an agarose gel (Figure 3) to probe the presence of the insert. Later the extracted plasmid was transformed on <i>E.coli</i> Rossetta (DE3).</p>
 ===User Reviews===
 <!-- DON'T DELETE --><partinfo>BBa_K2719007 StartReviews</partinfo>