Difference between revisions of "Part:BBa K2818001"
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<partinfo>BBa_K2818001 short</partinfo>
 
<partinfo>BBa_K2818001 short</partinfo>
  
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Similar to part<html><a href="https://parts.igem.org/Part:BBa_K2818001"> BBa_K2818002</a></html>, dPspCas13b-ADAR2<html><sub>DD</sub></html>, Cas13d-ADAR2DD(E488Q) is a similar fusion protein of a Type IV that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjunction with a guide RNA. NLS was fused to improve localisation to nucleus and hence RNA editing.  
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Similar to part<html><a href="https://parts.igem.org/Part:BBa_K2818001"> BBa_K2818002 (dPspCas13b-ADAR2<sub>DD</sub>)</a></html>, Cas13d-ADAR2DD(E488Q) is a similar fusion protein of a Type IV that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjunction with a guide RNA. NLS was fused to improve localisation to nucleus and hence RNA editing.  
  
 
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Revision as of 17:02, 17 October 2018


Cas13d-NLS-ADAR

Similar to part BBa_K2818002 (dPspCas13b-ADAR2DD), Cas13d-ADAR2DD(E488Q) is a similar fusion protein of a Type IV that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjunction with a guide RNA. NLS was fused to improve localisation to nucleus and hence RNA editing.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2935
    Illegal BamHI site found at 2965
    Illegal XhoI site found at 2410
    Illegal XhoI site found at 3666
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 223
  • 1000
    COMPATIBLE WITH RFC[1000]