Difference between revisions of "Part:BBa K2656019"

Revision as of 17:01, 17 October 2018

LuxI Coding Sequence

Part BBa_K2656013 is the Acyl-homoserine-lactone synthase (LuxI) coding sequence BBa_C0161 adapted to the Golden Braid grammar and so used with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/GB3 Golden Gate] assembly methods. It was codon optimized for E. coli.

LuxI coding sequence codifies for the 3OC6HSL (AHL) synthetase, so two signaling molecules of AHL interacts with two luxR proteins creating a complex which binds to the pLux promoter.

This part can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units in a one-step reaction with the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 8: / Line 8: @@
 LuxI coding sequence codifies for the 3OC6HSL (AHL) synthetase, so two signaling molecules of AHL interacts with two luxR proteins creating a complex which binds to the pLux promoter.
-It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units in a one-step reaction with the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol].
+This part can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units in a one-step reaction with the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol].
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