Difference between revisions of "Part:BBa K2842680"
Line 19: Line 19:
 
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|'''Related Device'''
 
|'''Related Device'''
−
|[https://parts.igem.org/Part:BBa_K1362101 BBa_K1362101]
+
|[https://parts.igem.org/Part:BBa_K2842690 BBa_K2842690]
 
|-
 
|-
 
|'''RFC standard'''
 
|'''RFC standard'''

Revision as of 16:55, 17 October 2018


Intein Monomer 1: RFP reporter flanked with orthogonal inteins

Intein Monomer 1
Function Standardised blue-white screening
Use in E. coli cells
Chassis Tested DH5α cells, BL21* cells
Abstraction Hierarchy Composite Device
Related Device BBa_K2842690
RFC standard RFC10,RFC21,RFC23
& RFC25 compatible
Backbone pSB1C3
Submitted by [http://2018.igem.org/Team:UCL UCL iGEM 2018]



Figure 1: BsaI digestions

(1) BsaI digested Intein Passenger BBa_K2842669
(2) BsaI digested RFP inteins BBa_K2842680
(3) BsaI digested GFP inteins BBa_K2842690
*edited to show relevant bands





















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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1165
    Illegal BsaI.rc site found at 28
    Illegal SapI site found at 903
    Illegal SapI.rc site found at 213