Difference between revisions of "Part:BBa K2623032"
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===Usage and Biology===
 
===Usage and Biology===
 
Under the induction of IPTG and Arabinose, this part can code SpyTag-OmpA, SpyCatcher-L7Ae and siRNA-C/Dbox to be encapsulated into OMVs through the isopeptide bond formed between SpyTag/SpyCatcher and the linkage between L7Ae and C/Dbox (Figure 1).
 
Under the induction of IPTG and Arabinose, this part can code SpyTag-OmpA, SpyCatcher-L7Ae and siRNA-C/Dbox to be encapsulated into OMVs through the isopeptide bond formed between SpyTag/SpyCatcher and the linkage between L7Ae and C/Dbox (Figure 1).
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<br><table><tr><th>
 
[[Image:SiOMVs.png|thumb|420px|Figure 1. Schematic illustration of our OMVs.]]</th><th></table>
 
[[Image:SiOMVs.png|thumb|420px|Figure 1. Schematic illustration of our OMVs.]]</th><th></table>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:49, 17 October 2018


A composite part linked by BBa_K2623024, BBa_K2623026 and BBa_K2623027

Summary

Our OMVs module is designed for siRNA delivery and then for cancer treatment. To demonstrate the conjugation of the L7Ae-C/Dbox inside OMVs, we linked ST/SC part with the box part (BBa_K2623027) to form our total circuit (BBa_K2623032).The box part can code siRNA target for Kras in human pancreatic ductal adenocarcinoma (PDAC) with a C/Dbox RNA structure to bind with L7Ae.

Usage and Biology

Under the induction of IPTG and Arabinose, this part can code SpyTag-OmpA, SpyCatcher-L7Ae and siRNA-C/Dbox to be encapsulated into OMVs through the isopeptide bond formed between SpyTag/SpyCatcher and the linkage between L7Ae and C/Dbox (Figure 1).


Figure 1. Schematic illustration of our OMVs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1965
    Illegal NheI site found at 1988
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3974
    Illegal BamHI site found at 3850
    Illegal BamHI site found at 4004
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 65
    Illegal AgeI site found at 3685
    Illegal AgeI site found at 4010
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3667