Difference between revisions of "Part:BBa K2765000"
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We obtained the promoter through PCR. Here is the electropherogram that we gained the promoters. | We obtained the promoter through PCR. Here is the electropherogram that we gained the promoters. | ||
| − | + | [[Image: T--BIT-China--iGEM2018-PartFeedback-realgel.png |center|400px|]] | |
Because we have no idea of the exact strength of these promoters, we chose both strong promoters and weak ones in this result and compared them with promoters that we have already known their strength. We tested downstream genes of Yap1 whose names were: ctt1, glr1, trx2, trr1, tsa1, sod2, gsh1, and gsh2, together with strong promoter gal1p and weak promoter msy1p.We obtained these promoters through PCR, and we ligated these promoters with gfp gene to express GFP protein and test their strength through fluorescence intensity. This is the first step to screen the proper promoter.The results of the fluorescence intensity are as follows. | Because we have no idea of the exact strength of these promoters, we chose both strong promoters and weak ones in this result and compared them with promoters that we have already known their strength. We tested downstream genes of Yap1 whose names were: ctt1, glr1, trx2, trr1, tsa1, sod2, gsh1, and gsh2, together with strong promoter gal1p and weak promoter msy1p.We obtained these promoters through PCR, and we ligated these promoters with gfp gene to express GFP protein and test their strength through fluorescence intensity. This is the first step to screen the proper promoter.The results of the fluorescence intensity are as follows. | ||
| − | + | [[Image: T--BIT-China--iGEM2018-PartsFeedback-experimentresult.png |center|400px|]] | |
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Revision as of 16:44, 17 October 2018
promoter of gene GLR1
This part consist of the promoter glr1(glr1p), which is an inducible promoter (the promoter of enolase) from Saccharomyces cerevisiae. In this project, we use it as the downstream gene promoter of Yap1 to turn on the expression of dCas9.We have 9 different promoters as option.
We obtained the promoter through PCR. Here is the electropherogram that we gained the promoters.
Because we have no idea of the exact strength of these promoters, we chose both strong promoters and weak ones in this result and compared them with promoters that we have already known their strength. We tested downstream genes of Yap1 whose names were: ctt1, glr1, trx2, trr1, tsa1, sod2, gsh1, and gsh2, together with strong promoter gal1p and weak promoter msy1p.We obtained these promoters through PCR, and we ligated these promoters with gfp gene to express GFP protein and test their strength through fluorescence intensity. This is the first step to screen the proper promoter.The results of the fluorescence intensity are as follows.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]